Biological Buffers

HEPES

CAS No.
7365-45-9
Catalogue ID
GTC-373

HEPES, CAS 7365-45-9. Source-derived identification, physical data and applications are provided for catalogue reference; request the current specification, COA and SDS for the required grade.

Catalogue aliases / related identifiers

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid); 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-; HEPES free acid; Good's buffer HEPES

Supplied catalogue source: GTC-373 · Page 127

Reference data — confirm before ordering or use

The information below is a structured migration from the supplied catalogue snapshot. It is not a current product specification, COA or SDS. Confirm the actual supplier, grade, batch documents, classification, packing and destination requirements for your enquiry.


Basic Information

CAS No.7365-45-9
EC No.230-907-9
Linear FormulaC8H18N2O4S
Molecular Weight238.30 g/mol
Appearancewhite crystalline powder
Melting Point234-238 °C (lit.)
Boiling pointNot available (typical reference)
DensityNot available (typical reference)
SolubilityFreely soluble in water (approx. 40 g/100 mL at 20 °C)
Storage ConditionsKeep in a tightly closed container in a cool, dry and well-ventilated place.
Chemical StructureMolecular Structure of HEPES

Key Applications

  • Biological buffer: HEPES is used to maintain stable pH conditions in biological and biochemical systems where controlled buffering performance around physiological range is required.

  • Cell culture reagent: The product is employed in cell culture media formulations to support consistent pH control during cell growth and experimental procedures.

  • Biochemical assay reagent: HEPES serves in biochemical assays where pH stability is essential for accurate enzyme activity and reaction monitoring.

  • Laboratory buffer reagent: It functions effectively in laboratory protocols for protein analysis and molecular biology workflows requiring reliable buffering.

  • Buffer formulation: HEPES is suitable for custom buffer preparation in research and process development applications across life science laboratories.


  • Consistent product quality: High-purity specifications and batch-to-batch consistency tailored for biochemical reagent applications.

  • Stable export supply: Reliable logistics support for sample, trial and bulk orders to meet global customer requirements.

  • SDS and COA support: Complete product documentation including SDS and COA provided upon request for quality and regulatory needs.

  • Flexible packaging options: Customized packing in 1 kg, 5 kg or 25 kg sizes with inner PE liners for safe storage and handling of the crystalline powder.

Before you order

Confirm the current commercial product record.

Include the required grade or purity, quantity, preferred packing, destination country/port and required document set.

  • 01Current specification
  • 02Batch or sample COA
  • 03Product- and supplier-specific SDS
  • 04Packing and shipping review
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